Data/hora: 21/05/2024 10:13:47

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	05/12/2022	
Data da última atualização: 	13/01/2023	
Autoria: 	PATROCINIO, T. A.; FERNANDES, C. A. de C.; MACEDO, G. C.; SILVA, A. D. da; BARBOSA, N. R. R.; QUINTAO, C. C. R.; RIBEIRO, M. E. da S.; CAMARGO, L. S. de A.	
Afiliação: 	TAIS APARECIDA PATROCINIO, UNIFENAS; CARLOS ANTÔNIO DE CARVALHO FERNANDES, UNIFENAS; GILSON COSTA MACEDO, Universidade Federal de Juiz de Fora; ADILSON DAVID DA SILVA, Universidade Federal de Juiz de Fora; NADIA REZENDE RAPOSO BARBOSA, Universidade Federal de Juiz de Fora; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; MARCELA EDUARDA DA SILVA RIBEIRO, UNIFENAS; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL.	
Título: 	RA33, an analogue of resveratrol, improves the development of in vitro-fertilized bovine embryos.	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Zygote, v. 30, n. 6, p. 891-894, 2022.	
DOI: 	https://doi.org/10.1017/S0967199422000430	
Idioma: 	Inglês	
Conteúdo: 	Oxidative stress is an undesirable effect of in vitro culture, which requires antioxidant supplementation. This study investigated the analogue of resveratrol (RA33) as an alternative to resveratrol, an antioxidant molecule, for the in vitro culture of in vitro-fertilized bovine embryos. The effect of different concentrations of RA33 on embryo development was evaluated and a comparison between RA33 and resveratrol was performed. The cleavage rate was higher (P < 0.05) with 2.5 &#956;M (69.0 ± 4.4%) than at 0, 0.1 or 0.5 uM RA33 (62.1 ± 2.0%, 60.7 ± 5.9% and 56.7 ± 5.8%, respectively). The blastocyst rates on days 7 and 8 post-fertilization with 2.5 &#956;M RA33 (19.4 ± 3.3% and 24.6 ± 3.3%, respectively) were higher (P < 0.05) than for 0 uM (12.4 ± 2.5% and 15.2±2.5%, respectively). When 2.5 uM RA33 was compared with 0.5 uM resveratrol, similar (P > 0.05) cleavage and blastocyst rates were found between them, but the cleavage rate was higher (P < 0.05) in the control (80.8 ± 3.4%) than for the resveratrol treatment (76.4 ± 3.6%). The numbers of apoptotic cells and the apoptotic index were lower (P < 0.05) with RA33 (6.5 ± 0.6 cells and 6.4 ± 0.7%, respectively) and resveratrol (5 ± 0.8 cells and 5.5 ± 1.0%, respectively) than in the control group (9.8 ± 1.2 cells and 8.9 ± 1.1%, respectively). In conclusion, RA33 can enhance the preimplantation development of in vitro-fertilized bovine embryos and be an alternative to resveratrol in embryo culture medium.	
Palavras-Chave: 	Apoptose; Fertilização in vitro.	
Thesagro: 	Antioxidante; Bovino; Cultura de Embrião; Embrião Animal; Fertilidade Animal.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (25894-1) - UPC - AP - DD -  -  -  - DIS